| 质粒名称: | 5'UTR CGG 99x FMR1-EGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 63091 |
| 存储实验室: | Nicolas Charlet-Berguerand |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | low Amp |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 室温 |
| 生长条件: | -- |
|
插入基因信息 |
|
| 插入基因名称: | expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | EGFP（插入件上的C端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | 5'UTR CGG 99x FMR1-EGFP was a gift from Nicolas Charlet-Berguerand (Addgene plasmid # 63091 ; http://n2t.net/addgene:63091 ; RRID:Addgene_63091) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 5'UTR CGG 99x FMR1-EGFP 4688 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1
as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These
repeats are in frame with EGFP..
ACCESSION .
VERSION .
KEYWORDS 5'UTR CGG 99x FMR1-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4688)
TITLE Charlet-Berguerand plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4688)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4688
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(1..105)
/gene="bla"
/label=AmpR promoter
primer_bind 173..191
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
enhancer 355..734
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 735..938
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 889..909
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 983..1002
/label=T7
/note="T7 promoter, forward primer"
promoter 983..1001
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1625..2341
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(1667..1688)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1928..1947)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2275..2296
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2372..2493
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2383..2402)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2437..2456
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2637..2659)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 2743..2883
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 2798..2817
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(2898..2915)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3069..3657)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3149..3168)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3828..4688)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4451..4470
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
181 tatcacgagg ccctttcgtc ttcaagaatt cagatcttca atattggcca ttagccatat
241 tattcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
301 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt
361 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
421 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
481 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
541 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
601 atcatatgcc aagtccgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
661 atgcccagta catgacctta cgggactttc ctacttggca gtacatctac gtattagtca
721 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
781 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
841 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
901 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
961 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
1021 gtttaaactt aagcttcgtc gacagggccc accgcggagg ccggcctcag tcaggcgctc
1081 agctccgttt cggtttcact tccggtggag ggccgcctct gagcgggcgg cgggccgacg
1141 gcgagcgcgg gcggcggcgg tgacggaggc gccgctgcca gggggcgtgc ggcagcgcgg
1201 cggcggcggc ggcggcggcg gcggcggcgg cggcggcgga ggcggcggcg gcggcggcgg
1261 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
1321 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
1381 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
1441 cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggctgggc
1501 ctcgagcgcc cgcagcccac ctctcggggg cgggctcccg gcgctagcag ggctgaagag
1561 aagatggagg agctggtggt ggaagtgcgg ggctccaatg gcgctttcta caaggcacct
1621 aggagtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
1681 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
1741 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
1801 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
1861 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
1921 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
1981 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
2041 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
2101 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
2161 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
2221 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
2281 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
2341 actctagagc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
2401 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
2461 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
2521 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
2581 ggtaacagct gcacgtggga tccctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
2641 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgagcagaca
2701 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
2761 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
2821 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
2881 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
2941 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
3001 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
3061 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
3121 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
3181 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
3241 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
3301 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
3361 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
3421 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
3481 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
3541 ccagttacct tcggaaanag agttggtagc tcttgatccg gcaaacaaac caccgctggt
3601 agcggtggtt tttttgtttg caagcagcag attacgcgca gaanaaaagg atctcaagaa
3661 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
3721 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
3781 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
3841 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
3901 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
3961 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
4021 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
4081 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
4141 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
4201 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
4261 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
4321 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
4381 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
4441 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
4501 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
4561 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
4621 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
4681 atactcat
//
